Our Publications

KDM6B protects T-ALL cells from NOTCH1-induced oncogenic stress.

Issa N, Bjeije H, Wilson ER, Krishnan A, Dunuwille WMB, Parsons TM, Zhang CR, Han W, Young AL, Ren Z, Ge K, Wang ES, Weng AP, Cashen A, Spencer DH, Challen GA.

Leukemia. 2023 Apr;37(4):728-740. doi: 10.1038/s41375-023-01853-9. Epub 2023 Feb 17. PMID: 36797416 Free PMC article.

DUSP6 mediates resistance to JAK2 inhibition and drives leukemic progression.

Kong T, Laranjeira ABA, Yang K, Fisher DAC, Yu L, Poittevin De La Frégonnière L, Wang AZ, Ruzinova MB, Fowles JS, Fulbright MC, Cox MJ, Celik H, Challen GA, Huang S, Oh ST.

Nat Cancer. 2023 Jan;4(1):108-127. doi: 10.1038/s43018-022-00486-8. Epub 2022 Dec 29. PMID: 36581736

Distinct Tumor Necrosis Factor Alpha Receptors Dictate Stem Cell Fitness versus Lineage Output in Dnmt3a-Mutant Clonal Hematopoiesis.

SanMiguel JM, Eudy E, Loberg MA, Young KA, Mistry JJ, Mujica KD, Schwartz LS, Stearns TM, Challen GA, Trowbridge JJ.

Cancer Discov. 2022 Dec 2;12(12):2763-2773. doi: 10.1158/2159-8290.CD-22-0086. PMID: 36169447 Free PMC article.

Effect of Clonal Hematopoiesis on Cardiovascular Disease in People Living with HIV.

Wiley B, Parsons TM, Burkart S, Young AL, Erlandson KM, Tassiopoulos KK, Wu K, Gurnett C, Presti RM, Bolton KL, Challen GA.

Exp Hematol. 2022 Oct;114:18-21. doi: 10.1016/j.exphem.2022.07.304. Epub 2022 Aug 6. PMID: 35940373

Txnip Enhances Fitness of Dnmt3a-Mutant Hematopoietic Stem Cells via p21.

Zhang CR, Ostrander EL, Kukhar O, Mallaney C, Sun J, Haussler E, Celik H, Koh WK, King KY, Gontarz P, Challen GA.

Blood Cancer Discov. 2022 May 5;3(3):220-239. doi: 10.1158/2643-3230.BCD-21-0132. PMID: 35394496 Free PMC article.

TET2 and DNMT3A Mutations Exert Divergent Effects on DNA Repair and Sensitivity of Leukemia Cells to PARP Inhibitors

Maifrede S, Le BV, Nieborowska-Skorska M, Golovine K, Sullivan-Reed K, Dunuwille WMB, Nacson J, Hulse M, Keith K, Madzo J, Caruso LB, Gazze Z, Lian Z, Padella A, Chitrala KN, Bartholdy BA, Matlawska-Wasowska K, Di Marcantonio D, Simonetti G, Greiner G, Sykes SM, Valent P, Paietta EM, Tallman MS, Fernandez HF, Litzow MR, Minden MD, Huang J, Martinelli G, Vassiliou GS, Tempera I, Piwocka K, Johnson N, Challen GA, Skorski T.

Cancer Res. 2021 Jul 2:canres.3761.2020.

A Humanized Animal Model Predicts Clonal Evolution and Therapeutic Vulnerabilities in Myeloproliferative Neoplasms

Celik H, Krug E, Zhang CR, Han W, Issa N, Koh WK, Bjeije H, Kukhar O, Allen M, Li T, Fisher DA, Fowles JS, Wong TN, Stubbs MC, Koblish HK, Oh ST, Challen GA.

Cancer Discov. 2021 Jun 30:candisc.1652.2020.

LKB1/ STK11 Is a Tumor Suppressor in the Progression of Myeloproliferative Neoplasms

Marinaccio C, Suraneni P, Celik H, Volk A, Wen QJ, Ling T, Bulic M, Lasho T, Koche RP, Famulare CA, Farnoud N, Stein B, Schieber M, Gurbuxani S, Root DE, Younger ST, Hoffman R, Gangat N, Ntziachristos P, Chandel NS, Levine RL, Rampal RK, Challen GA, Tefferi A, Crispino JD.

Cancer Discov. 2021 Jun;11(6):1398-1410.

Epigenomic regulation of human T-cell leukemia virus by chromatin-insulator CTCF

Cheng X, Joseph A, Castro V, Chen-Liaw A, Skidmore Z, Ueno T, Fujisawa JI, Rauch DA, Challen GA, Martinez MP, Green P, Griffith M, Payton JE, Edwards JR, Ratner L.

PLoS Pathog. 2021 May 21;17(5):e1009577.

Chronic infection drives Dnmt3a-loss-of-function clonal hematopoiesis via IFNγ signaling

Hormaechea-Agulla D, Matatall KA, Le DT, Kain B, Long X, Kus P, Jaksik R, Challen GA, Kimmel M, King KY.

Cell Stem Cell. 2021 Mar 16:S1934-5909(21)00108-9.

TGFβR-SMAD3 Signaling Induces Resistance to PARP Inhibitors in the Bone Marrow Microenvironment

Le BV, Podszywalow-Bartnicka P, Maifrede S, Sullivan-Reed K, Nieborowska-Skorska M, Golovine K, Yao JC, Nejati R, Cai KQ, Caruso LB, Swatler J, Dabrowski M, Lian Z, Valent P, Paietta EM, Levine RL, Fernandez HF, Tallman MS, Litzow MR, Huang J, Challen GA, Link D, Tempera I, Wasik MA, Piwocka K, Skorski T.

Cell Rep . 2020 Oct 6;33(1):108221.

Clonal hematopoiesis: mechanisms driving dominance of stem cell clones

Challen GA, Goodell MA.

Blood . 2020 Oct 1;136(14):1590-1598

Radiation induces iatrogenic immunosuppression by indirectly affecting hematopoiesis in bone marrow

Kapoor V, Collins A, Griffith K, Ghosh S, Wong N, Wang X, Challen GA, Krambs J, Link D, Hallahan DE, Thotala D. Oncotarget.

Oncotarget . 2020 May 12;11(19):1681-1690

Divergent Effects of Dnmt3a and Tet2 Mutations on Hematopoietic Progenitor Cell Fitness

Ostrander EL, Kramer AC, Mallaney C, Celik H, Koh WK, Fairchild J, Haussler E, Zhang CRC, Challen GA.

Stem Cell Reports . 2020 Apr 14;14(4):551-560.

Inflammatory cytokines promote clonal hematopoiesis with specific mutations in ulcerative colitis patients

Zhang CRC, Nix D, Gregory M, Ciorba MA, Ostrander EL, Newberry RD, Spencer DH, Challen GA.

Exp Hematol . 2019 Dec;80:36-41.e3.

IRAK4 mediates colitis-induced tumorigenesis and chemoresistance in colorectal cancer

Li Q, Chen Y, Zhang D, Grossman J, Li L, Khurana N, Jiang H, Grierson PM, Herndon J, DeNardo DG, Challen GA, Liu J, Ruzinova MB, Fields RC, Lim KH.

JCI Insight. 2019 Oct 3;4(19):e130867.

Kdm6b regulates context-dependent hematopoietic stem cell self-renewal and leukemogenesis

Mallaney C, Ostrander EL, Celik H, Kramer AC, Martens A, Kothari A, Koh WK, Haussler E, Iwamori N, Gontarz P, Zhang B, Challen GA.

Leukemia 2019 Oct;33(10):2506-2521

JARID2 Functions as a Tumor Suppressor in Myeloid Neoplasms by Repressing Self-Renewal in Hematopoietic Progenitor Cells

Celik H, Koh WK, Kramer AC, Ostrander EL, Mallaney C, Fisher DAC, Xiang J, Wilson WC, Martens A, Kothari A, Fishberger G, Tycksen E, Karpova D, Duncavage EJ, Lee Y, Oh ST, Challen GA.

Cancer Cell 2018 Nov 12;34(5):741-756

An Activating Mutation of Interferon Regulatory Factor 4 (IRF4) in Adult T-Cell Leukemia

Cherian MA, Olson S, Sundaramoorthi H, Cates K, Cheng X, Harding J, Martens A, Challen GA, Tyagi M, Ratner L, Rauch D

J Biol Chem 2018 May 4;293(18):6844-6858

Loss of Dnmt3a Immortalizes Hematopoietic Stem Cells in Vivo

Jeong M*, Park HJ*, Celik H*, Ostrander EL, Reyes JM, Guzman A, Rodriguez B, Lei Y, Lee Y, Ding L, Guryanova OA, Li W, Goodell MA, Challen GA

Cell Rep 2018 Apr 3;23(1):1-10 *Co-first author

Breast and Pancreatic Cancer Interrupt IRF8-Dependent Dendritic Cell Development to Overcome Immune Surveillance

Meyer MA, Baer JM, Knolhoff BL, Nywening TM, Panni RZ, Su X, Weilbaecher KN, Hawkins WG, Ma C, Fields RC, Linehan DC, Challen GA, Faccio R, Aft RL, DeNardo DG

Nat Commun 2018 Mar 28;9(1):1250

Protein Kinase C Epsilon Is a Key Regulator of Mitochondrial Redox Homeostasis in Acute Myeloid Leukemia

Di Marcantonio D, Martinez E, Sidoli S, Vadaketh J, Nieborowska-Skorska M, Gupta A, Meadows JM, Ferraro F, Masselli E, Challen GA, Milsom MD, Scholl C, Frohling S, Balachandran S, Skorski T, Garcia BA, Mirandola P, Gobbi G, Garzon R, Vitale M, Sykes SM

Clin Cancer Res 2018 Feb 1;24(3):608-618

A TFIID-SAGA Perturbation That Targets MYB and Suppresses Acute Myeloid Leukemia

Xu Y, Milazzo JP, Somerville TDD, Tarumoto Y, Huang YH, Ostrander EL, Wilkinson JE, Challen GA, Vakoc CR

Cancer Cell 2018 Jan 8;33(1):13-28e8

The GNAS(R201C) Mutation Associated With Clonal Hematopoiesis Supports Transplantable Hematopoietic Stem Cell Activity

Ostrander EL, Koh WK, Mallaney C, Kramer AC, Wilson WC, Zhang B, Challen GA

Exp Hematol 2018 Jan;57:14-20

Dnmt3a Regulates T-Cell Development and Suppresses T-ALL Transformation

Kramer AC, Kothari A, Wilson WC, Celik H, Nikitas J, Mallaney C, Ostrander EL, Eultgen E, Martens A, Valentine MC, Young AL, Druley TE, Figueroa ME, Zhang B, Challen GA

Leukemia 2017 Nov;31(11):2479-2490

Clonal Haematopoiesis Harbouring AML-Associated Mutations Is Ubiquitous in Healthy Adults

Young AL, Challen GA, Birmann BM, Druley TE

Nat Commun 2016 Aug 22;7:12484

DNMT3A Loss Drives Enhancer Hypomethylation in FLT3-ITD-Associated Leukemias

Yang L, Rodriguez B, Mayle A, Park HJ, Lin X, Luo M, Jeong M, Curry CV, Kim SB, Ruau D, Zhang X, Zhou T, Zhou M, Rebel VI, Challen GA, Gottgens B, Lee JS, Rau R, Li W, Goodell MA

Cancer Cell 2016 Jun 13;29(6):922-934

Reprogrammable CRISPR/Cas9-Based System for Inducing Site-Specific DNA Methylation

McDonald J*, Celik H*, Rois LE, Fishberger G, Fowler T, Rees R, Kramer A, Martens A, Edwards JR, Challen GA

Biol Open 2016 Jun 15;5(6):866-74 *Co-first author

Enforced Differentiation of Dnmt3a-Null Bone Marrow Leads to Failure With c-Kit Mutations Driving Leukemic Transformation

Celik H, Mallaney C, Kothari A, Ostrander EL, Eultgen E, Martens A, Miller CA, Hundal J, Klco JM, Challen GA

Blood 2015 Jan 22;125(4):619-28

Dnmt3a Loss Predisposes Murine Hematopoietic Stem Cells to Malignant Transformation

Mayle A, Yang L, Rodriguez B, Zhou T, Chang E, Curry CV, Challen GA, Li W, Wheeler D, Rebel VI, Goodell MA

Blood 2015 Jan 22;125(4):629-38

Type II Interferon Promotes Differentiation of Myeloid-Biased Hematopoietic Stem Cells

Matatall KA, Shen CC, Challen GA, King KY

Stem Cells 2014 Nov;32(11):3023-30

Dnmt3a and Dnmt3b Have Overlapping and Distinct Functions in Hematopoietic Stem Cells

Challen GA, Sun D, Mayle A, Jeong M, Luo M, Rodriguez B, Mallaney C, Celik H, Yang L, Xia Z, Cullen S, Berg J, Zheng Y, Darlington GJ, Li W, Goodell MA

Cell Stem Cell 2014 Sep 4;15(3):350-64

Clonal-Level Responses of Functionally Distinct Hematopoietic Stem Cells to Trophic Factors

Mallaney C, Kothari A, Martens A, Challen GA

Exp Hematol 2014 Apr;42(4):317-327e2

Large Conserved Domains of Low DNA Methylation Maintained by Dnmt3a

Jeong M, Sun D, Luo M, Huang Y, Challen GA, Rodriguez B, Zhang X, Chavez L, Wang H, Hannah R, Kim SB, Yang L, Ko M, Chen R, Gottgens B, Lee JS, Gunaratne P, Godley LA, Darlington GJ, Rao A, Li W, Goodell MA

Nat Genet 2014 Jan;46(1):17-23

Dnmt3a Is Essential for Hematopoietic Stem Cell Differentiation

Challen GA, Sun D, Jeong M, Luo M, Jelinek J, Berg JS, Bock C, Vasanthakumar A, Gu H, Xi Y, Liang S, Lu Y, Darlington GJ, Meissner A, Issa JP, Godley LA, Li W, Goodell MA

Nat Genet 2011 Dec 4;44(1):23-31

Investigating the Role of Hematopoietic Stem and Progenitor Cells in Regulating the Osteogenic Differentiation of Mesenchymal Stem Cells in Vitro

Liao J, Hammerick KE, Challen GA, Goodell MA, Kasper FK, Mikos AG

J Orthop Res 2011 Oct;29(10):1544-53

Distinct Hematopoietic Stem Cell Subtypes Are Differentially Regulated by TGF-Beta1

Challen GA, Boles NC, Chambers SM, Goodell MA

Cell Stem Cell 2010 Mar 5;6(3):265-78

Runx1 Isoforms Show Differential Expression Patterns During Hematopoietic Development but Have Similar Functional Effects in Adult Hematopoietic Stem Cells

Challen GA, Goodell MA

Exp Hematol 2010 May;38(5):403-16

Join Our Team

Challen Lab actively recruits motivated and enthusiastic post-docs, Ph.D. students and technicians to work and assist on projects related to stem cells, epigenetics and leukemia research!

Challen Lab trainees have a 100% success rate for acquiring awards and fellowships!

Please send CV/resume, references, and research statements to Dr. Grant Challen